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Image Search Results
Journal: bioRxiv
Article Title: An endothelial specific mouse model for the capillary malformation mutation Gnaq p.R183Q
doi: 10.1101/2025.03.17.643802
Figure Lengend Snippet: (A) Single channel and merged images of immunofluorescent staining of mutant intestines or ear skin for CD31, GFP and the mural cell markers PDGFRb, NG-2 and alpha-SMA. Note that intestinal lesions stain stronger for PDGFRb and NG-2, while alpha-SMA staining is irregular in the GFP positive dorsal ear skin vessel. (B) Immunofluorescent staining of human CM and control skin for NG-2. Note that enlarged vessels have multiple layers of NG-2 expressing pericytes.
Article Snippet: The following primary antibodies were used: rat anti-CD31 (1/500, BD Pharmigen, 553370); chicken anti-GFP (1/1000 Abcam, ab13970); rabbit anti-PDGFRb (1/250, Cell Signaling Technologies (CST), 3169); rabbit anti-NG2 (1/250, Sigma, AB5320);
Techniques: Staining, Mutagenesis, Control, Expressing